Coding

Part:BBa_K3513004:Design

Designed by: Huandi Xu   Group: iGEM20_NFLS   (2020-10-23)


exoglucanase with signal peptide, linker and promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 637
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 102
    Illegal NgoMIV site found at 270
    Illegal NgoMIV site found at 643
    Illegal NgoMIV site found at 1145
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 690
    Illegal SapI.rc site found at 773


Design Notes

Bind with promoter J23119


Source

Origin source of endoglucanase: Streptomyces Coelicolor. But the team engineered it.


References